Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

Ingrid M E Desar; Maaike de Boer; Corina C P M Bens; Jan A Jacobs; Johan W Mouton; Anton S M Dofferhoff; Corné H W Klaassen

doi:10.1016/j.mimet.2008.06.022

Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis

J Microbiol Methods. 2008 Oct;75(2):372-4. doi: 10.1016/j.mimet.2008.06.022. Epub 2008 Jul 3.

Authors

Ingrid M E Desar¹, Maaike de Boer, Corina C P M Bens, Jan A Jacobs, Johan W Mouton, Anton S M Dofferhoff, Corné H W Klaassen

Affiliation

¹ Department of Internal Medicine, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.

PMID: 18652853
DOI: 10.1016/j.mimet.2008.06.022

Abstract

A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.

Publication types

Evaluation Study

MeSH terms

Bacteremia / microbiology
Bacterial Typing Techniques*
DNA Probes
DNA, Ribosomal Spacer / analysis
DNA, Ribosomal Spacer / genetics
Humans
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
Reproducibility of Results
Species Specificity
Staphylococcal Infections / microbiology
Staphylococcus / classification*
Staphylococcus / genetics
Staphylococcus / isolation & purification
Time Factors
Transition Temperature*

Substances

DNA Probes
DNA, Ribosomal Spacer
RNA, Ribosomal, 16S