Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Neuropsychopharmacology. 2009 Mar;34(4):867-73. doi: 10.1038/npp.2008.108. Epub 2008 Jul 23.

Increased OPRM1 DNA methylation in lymphocytes of methadone-maintained former heroin addicts.

Author information

  • 1Laboratory of the Biology of the Addictive Diseases, The Rockefeller University, New York, NY 10065, USA. nielsen@rockefeller.edu

Erratum in

  • Neuropsychopharmacology. 2009 Mar;34(4):1087.

Abstract

The mu-opioid receptor is the site of action of opiates and opioids. We examined whether there are differences in cytosine:guanine (CpG) dinucleotide methylation in the OPRM1 promoter between former heroin addicts and controls. We analyzed methylation at 16 CpG dinucleotides in DNA obtained from lymphocytes of 194 Caucasian former severe heroin addicts stabilized in methadone maintenance treatment and 135 Caucasian control subjects. Direct sequencing of bisulfite-treated DNA showed that the percent methylation at two CpG sites was significantly associated with heroin addiction. The level of methylation at the -18 CpG site was 25.4% in the stabilized methadone-maintained former heroin addicts and 21.4% in controls (p=0.0035, generalized estimating equations (GEE); p=0.0077, t-test; false discovery rate (FDR)=0.048), and the level of methylation at the +84 CpG dinucleotide site was 7.4% in cases and 5.6% in controls (p=0.0095, GEE; p=0.0067, t-test; FDR=0.080). Both the -18 and the +84 CpG sites are located in potential Sp1 transcription factor-binding sites. Methylation of these CpG sites may lead to reduced OPRM1 expression in the lymphocytes of these former heroin addicts.

PMID:
18650805
[PubMed - indexed for MEDLINE]
PMCID:
PMC2778040
Free PMC Article

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Write to the Help Desk