Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7

Biosens Bioelectron. 2008 Dec 1;24(4):558-65. doi: 10.1016/j.bios.2008.06.019. Epub 2008 Jun 20.

Abstract

This work demonstrated the feasibility of detecting 250zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / genetics*
  • Equipment Design
  • Equipment Failure Analysis
  • Escherichia coli O157 / genetics*
  • Escherichia coli O157 / isolation & purification*
  • Magnetics / instrumentation*
  • Quantum Dots*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / instrumentation*
  • Staining and Labeling / methods

Substances

  • DNA, Bacterial