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Curr Opin Chem Biol. 2008 Aug;12(4):448-56. doi: 10.1016/j.cbpa.2008.06.028. Epub 2008 Jul 21.

SELEX with modified nucleotides.

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  • 1Archemix Corp., 300 Third Street, Cambridge, MA 02142, USA. keefe@archemix.com

Abstract

Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucleotide libraries containing modified nucleotides are increasingly being used for selection. Principally, the choice of modifications aims to increase aptamer potency by enhancing nuclease-resistance, or increasing target affinity by providing more target recognition functionality or generating more stable aptamer structures.

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