Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Lett. 2008 Jul 15;33(14):1596-8.

Field-based angle-resolved light-scattering study of single live cells.

Author information

  • 1G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, USA. wonshik@mit.edu

Abstract

We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.

PMID:
18628809
[PubMed - indexed for MEDLINE]
PMCID:
PMC2833213
Free PMC Article

Images from this publication.See all images (3)Free text

Fig. 1
Fig. 2
Fig. 3
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Optical Society of America Icon for PubMed Central
    Loading ...
    Write to the Help Desk