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J Postgrad Med. 2008 Jul-Sep;54(3):181-5.

Comparison of a dipstick enzyme-linked immunosorbent assay with commercial assays for detection of Japanese encephalitis virus-specific IgM antibodies.

Author information

  • 1Division of Virology, Defense R and D Establishment, Jhansi Road, Gwalior, MP - 474 002, India. ambujshrivastava@hotmail.com

Abstract

BACKGROUND:

Japanese encephalitis (JE) is a major public health concern in Asia including India. Objectives: To evaluate an in-house developed dipstick enzyme-linked immunosorbent assay (ELISA) test vis-à-vis two commercial kits for detection of JE virus-specific IgM antibodies.

SETTING AND DESIGN:

Comparative study carried out in Research and Development centre.

MATERIALS AND METHODS:

A total of 136 specimens comprising 84 serum and 52 CSF samples were tested by in-house dipstick ELISA, Pan-Bio IgM capture ELISA (Pan-Bio, Australia) and JEV CheX IgM capture ELISA (XCyton, India).

RESULTS:

The overall agreement among all three tests was found to be 92% with both serum and cerebrospinal fluid (CSF) samples. The sensitivity of the dipstick ELISA was found to be 91% with serum and 89% with CSF samples respectively. The specificity of the dipstick ELISA with reference to both commercial assays was found to be 100% in serum and CSF samples in this study.

CONCLUSIONS:

The in-house dipstick ELISA with its comparable sensitivity and specificity can be used as a promising test in field conditions since it is simple, rapid and requires no specialized equipment.

PMID:
18626163
[PubMed - indexed for MEDLINE]
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