Cytotoxicity of oxidised lipids in cultured colonal human intestinal cancer cells (caco-2 cells).

Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.

In this study, we investigated the extent of the cytotoxicity effect of oxidised lipids and whether tea catechins namely (-)epigallocatechin-3 gallate (EGCG) decreased lipid peroxidation in caco-2 cells. Cells treated with 0-100 microg/ml fish oil or methyl linoleate (ML) oxidised by UV irradiation for 24 h, 48 h and 72 h, indicated a substantial decrease in cell viability especially in samples treated with 100 microg/ml oxidised lipid. Addition of malondialdehyde (MDA) and hydroxynonenal (50 microM) also reduced cell viability. Using EGCG (50 microM) increased the viability of cells treated with 24 h oxidised mackerel oil (72% live and 28% dead) compared with 48 h oxidised mackerel oil (89% live and 11% dead) and 72 h oxidised mackerel oil (71% live and 29% dead) as monitored by the MTT assay. Apoptosis of caco-2 cells by oxidised fish oil and ML and protection by EGCG was confirmed using fluorescence microscopy and caspase-3 presence by Western blotting.

PMID: 18625293 [PubMed - indexed for MEDLINE]