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J Mater Sci Mater Med. 2008 Dec;19(12):3525-33. doi: 10.1007/s10856-008-3500-8. Epub 2008 Jul 15.

PEG-grafted chitosan nanoparticles as an injectable carrier for sustained protein release.

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  • 1Key Laboratory of Functional Polymer Materials, Ministry Education, and Institute of Polymer Chemistry, Nankai University, 94 Weijin Road, Tianjin, 300071, China. zhangxinge@nankai.edu.cn


The development of injectable nanoparticulate "stealth" carriers for protein delivery is a major challenge. The objective of this work was to investigate the possibility of achieving the controlled release of a model protein, insulin, from PEG-grafted chitosan (PEG-g-chitosan) nanoparticles (mean diameter 150-300 nm) prepared by the ion gelation method. Insulin was efficiently incorporated into the nanoparticles, and reached as high as 38%. In vitro release showed that it could control the insulin release by choosing the composition, loading and release temperature. We observed that the composition of the nanoparticle surface (C/O ratio) increased from 2.40 to 3.23, with an increase in the incubation time. Therefore, we concluded that during this time, insulin release from PEG-g-chitosan nanoparticles followed a diffusion mechanism in which erosion was negligible. The experiments also demonstrated that PEG-g-chitosan helped to maintain the natural structure of the protein entrapped in the nanoparticles.

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