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J Chromatogr A. 2008 Aug 15;1202(1):107-10. doi: 10.1016/j.chroma.2008.06.032. Epub 2008 Jul 1.

Use of cyclohexanedimethanol as a nonflammable organic solvent for industrial scale reversed phase chromatography.

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  • 1Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA. alan.k.hunter@pfizer.com

Abstract

This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-I(Milano). Cyclohexanedimethanol has low viscosity in water, enabling the use of conventional low pressure process scale chromatography columns and hardware. Results from pilot scale manufacturing using a 30 cm diameter CG71M packed column indicate that a 5.7 log reduction in E. coli host cell protein with >80% yield can be obtained for the purification of apolipoprotein A-I(Milano).

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