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Growth Horm IGF Res. 2009 Feb;19(1):43-50. doi: 10.1016/j.ghir.2008.06.001. Epub 2008 Jul 9.

A determination of the pre-analytical storage conditions for insulin like growth factor-I and type III procollagen peptide.

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  • 1Endocrinology and Metabolism Sub-Division, Developmental Origins of Adult Health and Disease Division, School of Medicine, University of Southampton, Southampton General Hospital, Southampton, United Kingdom. R.I.G.Holt@soton.ac.uk

Abstract

OBJECTIVE:

IGF-I and type III procollagen (P-III-P) have been proposed as markers to detect GH abuse. This study aims to determine whether the pre-analytical storage temperature or delayed centrifugation affect the measured IGF-I and P-III-P concentrations.

DESIGN:

Observational study.

SETTING:

Wellcome Trust Clinical Research Facility, Southampton.

SUBJECTS:

Nineteen healthy volunteers.

INTERVENTION:

Blood was collected into bottles containing a clotting agent, lithium heparin or EDTA. One sample from each group was centrifuged and stored at -80 degrees C (control sample). The remaining samples from each group were stored as either serum or whole blood at 4 degrees C or room temperature for up to five days prior to storage at -80 degrees C.

OUTCOME MEASURES:

IGF-I and P-III-P.

RESULTS:

The storage temperature or timing of centrifugation did not appear to affect IGF-I concentration. In contrast, the measured P-III-P concentration rose by 6.5-7% per day in clotted and lithium heparin samples when stored as whole blood (p<0.006) or serum (6.2-6.5% per day) at room temperature (p<0.001). P-III-P did not change when the samples were stored at 4 degrees C. Although collection into EDTA inhibited the rise in P-III-P, the baseline measured values were significantly higher than in other media and spiking experiments demonstrated that EDTA exerted a significant matrix effect on the assay.

CONCLUSION:

While the optimum collection method is immediate centrifugation and storage at -80 degrees C, it would seem acceptable to store serum or clotted blood samples at 4 degrees C, but not ambient temperature, for up to five days. It is incumbent on the anti-doping authorities to provide facilities to allow this.

[PubMed - indexed for MEDLINE]
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