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1: Virol J. 2008 Jul 10;5:79.Click here to read Click here to read Links

Genetic heterogeneity of L-Zagreb mumps virus vaccine strain.

Kosutic-Gulija T, Forcic D, Santak M, Ramljak A, Mateljak-Lukacevic S, Mazuran R.

Department for Research and Development, Institute of Immunology Inc, Rockefeller Street 10, Zagreb, Croatia. tgulija@imz.hr

BACKGROUND: The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous. RESULTS: We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo. CONCLUSION: L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.

PMID: 18616793 [PubMed - indexed for MEDLINE]

PMCID: PMC2481254