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J Clin Microbiol. 2008 Sep;46(9):3116-8. doi: 10.1128/JCM.00440-08. Epub 2008 Jul 9.

Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.

Author information

  • 1Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jwinchell@cdc.gov

Abstract

We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.

PMID:
18614663
[PubMed - indexed for MEDLINE]
PMCID:
PMC2546712
Free PMC Article
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