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1: J Clin Microbiol. 2008 Sep;46(9):3107-9. Epub 2008 Jul 9.Click here to read Click here to read Links

MVPlex assay for direct detection of methicillin-resistant Staphylococcus aureus in Naris and other swab specimens.

Podzorski RP, Li H, Han J, Tang YW.

Department of Pathology, Waukesha Memorial Hospital, Waukesha, Wisconsin 53188, USA. raymond.podzorski@phci.org

We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the "gold standard," the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively.

PMID: 18614646 [PubMed - in process]

PMCID: PMC2546723