(A) Diagram depicting the procedure followed for the high resolution MNase assay. S2 cells are heat shocked for 0 (dark blue), 5 (light blue), 30 (green), 60 (orange), or 120 seconds (red) (colors refer to 1C), immediately cooled to room temperature and crosslinked, and their chromatin is isolated. Purified DNA products from samples are treated with 0 or 500 U of MNase and used for qPCR.
(B) Diagram showing the PCR amplicons used at the Hsp70Ab gene. PCR products are 100±5 bp in size and are spaced 30±6 bp apart. The mRNA-encoding unit is shown in black. The gene nucleotide location, corresponding to panel C, is numbered below with the HSEs, TSS, and PolyA site (at +2343) indicated.
(C) The HS time course chromatin profile of Hsp70 is determined by normalizing the amount of the MNase digested PCR product to that of the undigested product using the ΔC(t) method (y-axis), which is plotted against the gene nucleotide location (x-axis). Values from overlapping primer sets are averaged. The x-axis represents base pair units with 0 being the TSS. Lines represent the average of 3 separate experiments with error bars omitted for clarity.

(A) ChIP at Hsp70 for HSF and Rpb3 (Pol II) with and without 125 µM of the transcription inhibitor DRB before (NHS, black bars and NHS+DRB, dark gray bars) and during HS (2’HS, white bars and 2’HS+DRB, light gray bars). The y-axis represents the percent of input material immunoprecipitated. x-axis values represent base pair units centered on the HSEs (−154), the Pol II pause site (+58), a downstream region (+1702), and an intergenic region outside the scs and scs’ regions. Error bars represent the SEM of 3 independent experiments.
(B) MNase protection profile of NHS with DRB (dark gray), 2’HS with DRB (light gray), and 2’HS (medium gray) as in Figure 1C. Lines represent the average of 3 separate experiments. Error bars representing the SEM are plotted just for the 2’HS+DRB line for clarity.
(C) ChIP as described in (A) except with and without 10 mM of sodium salicylate before (NHS, black bars and NHS+salicylate, dark gray bars) and during HS (20’HS, white bars and 20’HS+salicylate, light gray bars). Error bars represent the SEM of 3 independent experiments.
(D) MNase protection profile as in (B) with NHS (black), NHS+salicylate (dark gray), and 1’HS (light gray). Lines represent the average of 3 separate experiments. Error bars representing the SEM are plotted just for the NHS+salicylate line for clarity.

MNase protection profile as in Figure 1C comparing the chromatin architecture following an RNAi depletion of either (A) HSF, (B) GAF, or (C) PARP (black lines) in comparison to a control RNAi depletion of LacZ (gray lines) after a 2’HS at Hsp70. Each line represents the average of 3 independent experiments with error bars representing the SEM. Western blots showing corresponding knockdown of HSF, GAF, or PARP are shown to the right of each figure with TFIIS used as a loading control and a serial dilution of LacZ RNAi to quantify each knockdown.

Histone density across the Hsp70 gene detected by ChIP using an H3 antibody. S2 cells are heat shocked for 0 (dark blue), 5 (light blue), 30 (green), 60 (orange), or 120 seconds (red). The y-axis represents the percent of input material immunoprecipitated. Error bars represent the SEM of 3 independent experiments. The x-axis represents base pair units along the Hsp70 gene with 0 as the TSS; each number represents the center of the PCR amplicon. The intergenic region represents a region 32 kb downstream of Hsp70Bc that does not change upon HS.

(A –D) MNase HS time course protection as in Figure 1C flanking the scs and scs’ insulators. The 87A HS locus shown above depicts the 4 sites analyzed flanking scs and scs’. Regions A and C are outside and B and D are inside of the scs and scs’ insulators respectively. Regions A–D also correspond to A–D in the graphs. The x-axis of all 4 graphs uses the TSS of the Hsp70Ab copy as the 0 point. Each line represented is the average of 3 independent experiments. Error bars are omitted for clarity but the SEM from 3 independent experiments is less than ±0.06.

(A) MNase protection profile of Hsp70 as in Figure 1C comparing the chromatin architecture of S2 cells treated (black line) or not treated (gray line) with the PARP enzymatic inhibitor PJ34 after a 2’HS. Each line represents the average of 3 independent experiments with error bars representing the SEM.
(B) Hsp70 mRNA levels following a 2, 5, and 20 minute HS were measured for S2 cells RNAi depleted of LacZ (black) or PARP (gray). Hsp70 expression levels were measured by oligo dT primed reverse transcription followed by qPCR using specific Hsp70Ab primers. Hsp70 mRNA levels are normalized to the Rp49 gene with error bars representing the SEM of 3 replicates.