Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Proteome Res. 2008 Aug;7(8):3543-55. doi: 10.1021/pr800286z. Epub 2008 Jul 10.

Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: bypassing formalin fixation.

Author information

  • 1Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-8575, USA. pierre.chaurand@vanderbilt.edu

Abstract

Imaging mass spectrometry is becoming a key technology for the investigation of the molecular content of biological tissue sections in direct correlation with the underlying histology. Much of our work has been done with fresh-frozen tissue sections that has undergone minimal protein degradation between the time a tissue biopsy is sampled and the time it is snap-frozen so that no preserving or fixing agents need to be added to the frozen biopsy. However, in many sampling environments, immediate flash freezing may not be possible and so we have explored the use of ethanol-preserved, paraffin-embedded tissue specimens for proteomic analyses. Solvent-only preserved tissue specimens provide long-term preservation at room temperature, generation of high quality histological sections and little if any chemical alteration of the proteins. Using mouse organs, several key steps involved in the tissue dehydration process have been investigated to assess the potential of such preserved specimens for profiling and imaging mass spectrometry investigations.

PMID:
18613713
[PubMed - indexed for MEDLINE]
PMCID:
PMC2927987
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Write to the Help Desk