Identification of a cryptic type III polyketide synthase (1,3,6,8-tetrahydroxynaphthalene synthase) from Streptomyces peucetius ATCC 27952

Mol Cells. 2008 Oct 31;26(4):362-7. Epub 2008 Jul 7.

Abstract

We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydroxynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / chemistry
  • Acyltransferases / isolation & purification*
  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Sequence Data
  • Naphthols / chemistry
  • Naphthoquinones / chemistry
  • Phenotype
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Spectrometry, Mass, Electrospray Ionization
  • Streptomyces / enzymology*

Substances

  • 1,3,6,8-tetrahydroxynaphthalene
  • Naphthols
  • Naphthoquinones
  • flaviolin
  • Acyltransferases
  • flavanone synthetase