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Nucleic Acids Res. 2008 Aug;36(13):4474-87. doi: 10.1093/nar/gkn411. Epub 2008 Jul 4.

p100 increases AT1R expression through interaction with AT1R 3'-UTR.

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  • 1Research Program for Molecular Medicine, Biomedicum Helsinki, University of Helsinki, Finland. kpaukku@mappi.helsinki.fi

Abstract

p100 protein (SND1, Tudor-SN) is a multifunctional protein that functions as a co-activator for several transcription factors, has a role in mRNA processing and participates in RNAi-induced silencing complex (RISC) with yet unknown function. In this study we identified a novel function for p100 as a regulator of angiotensin II type 1 receptor (AT1R) expression. The binding of p100 to AT1R 3'-untranslated region (3'-UTR) via staphylococcal nuclease-like (SN-like) domains increased receptor expression by decreasing the rate of mRNA decay and enhancing its translation. Overexpression of p100 increased AT1R expression, whereas decrease in p100 binding to 3'-UTR either by p100 silencing or by the deletion of p100 binding site downregulated receptor expression. The effect of p100 through AT1R 3'-UTR was independent of Argonaute2 (Ago2), a known p100 partner, and was thus RISC-independent. Nucleotides 118 to 120 of the AT1R 3'-UTR were found to be critical for the binding of p100 to 3'-UTR. In summary, p100 is a multifunctional regulator of gene expression that regulates transcription, mRNA maturation, and as described in this article, also mRNA stability and translation.

PMID:
18603592
[PubMed - indexed for MEDLINE]
PMCID:
PMC2490763
Free PMC Article
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