An actinomycete, designated strain TJ2-2(T), was isolated from soil collected from a mangrove forest in Pattaloong Province, Thailand, and was subjected to morphological and chemotaxonomic analysis and phylogenetic investigation based on 16S rRNA gene sequences. The data from these analyses indicated that the novel strain should be classified as a member of the genus Micromonospora and that the closest relative was Micromonospora olivasterospora DSM 43868(T) (98.7 % gene sequence similarity). The DNA-DNA hybridization data and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain TJ2-2(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora pattaloongensis sp. nov. is proposed. The type strain is TJ2-2(T) (=JCM 12833(T)=TISTR 1559(T)).