The sorption of As(V) from aqueous solution onto live and pretreated biomass of Aspergillus fumigatus was studied. The studies on optimization of contact time, adsorbent dosage and pH showed that the FeCl(3) treated and FeSO(4) treated biomass had the maximum capacity to adsorb As(V) while acid treated biomass was found to be minimum. Adsorption parameters were determined using both Langmuir and Freundlich isotherm models. The maximum adsorption capacity of 0.054 mg/g was observed in FeCl(3) treated and FeSO(4) treated biomasses. Column mode studies were conducted using FeSO(4) treated biomass to compare its efficacy with batch mode to adsorb As(V). Batch mode experiments proved to be efficient. Desorption studies were also carried out with dilute sodium hydroxide to recover both the adsorbent and adsorbate.