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1: Eur J Histochem. 2008 Apr-Jun;52(2):135-9.Links

Processing scarce biological samples for light and transmission electron microscopy.

Taupin P.

National Neuroscience Institute, Singapore. obgpjt@nus.edu.sg

Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-embedding the biological samples, suspensions or pellets, in bovine serum albumin (BSA) and bis-acrylamide (BA), cross-linked and polymerized. This preparation provides a simple and reproducible technique to process biological materials, present in limited quantities that can not be amplified, for light and transmission electron microscopy.

PMID: 18591160 [PubMed - indexed for MEDLINE]