My NCBISign In

Display Settings:

Format

Send to:

Choose Destination
    Cell. 2008 Jun 27;133(7):1277-89.

    Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites.

    Noyes MB, Christensen RG, Wakabayashi A, Stormo GD, Brodsky MH, Wolfe SA.

    Source

    Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA 01605, USA.

    Abstract

    We describe the comprehensive characterization of homeodomain DNA-binding specificities from a metazoan genome. The analysis of all 84 independent homeodomains from D. melanogaster reveals the breadth of DNA sequences that can be specified by this recognition motif. The majority of these factors can be organized into 11 different specificity groups, where the preferred recognition sequence between these groups can differ at up to four of the six core recognition positions. Analysis of the recognition motifs within these groups led to a catalog of common specificity determinants that may cooperate or compete to define the binding site preference. With these recognition principles, a homeodomain can be reengineered to create factors where its specificity is altered at the majority of recognition positions. This resource also allows prediction of homeodomain specificities from other organisms, which is demonstrated by the prediction and analysis of human homeodomain specificities.

    Comment in

    PMID:
    18585360
    [PubMed - indexed for MEDLINE]
    PMCID: PMC2478728
    Free PMC Article

    Images from this publication.See all images (7) Free text

    Figure 7
    Figure 6
    Figure 4
    Figure 2
    Figure 5
    Figure 1
    Figure 3

    Publication Types, MeSH Terms, Substances, Grant Support

    Publication Types

    MeSH Terms

    Substances

    Grant Support

    LinkOut - more resources

    Full Text Sources

    Other Literature Sources

      Supplemental Content

      Click here to read Click here to read

      Related citations

      See reviews... See all...

      Cited by 47 PubMed Central articles

      See all...

      Chemical compound information

      • Chemical compound molecule image DNA
        MW: 523.36 g/mol
        MF: C15H31N3O13P2

      Related information

      • Related Citations
        Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
      • Compound (MeSH Keyword)
        PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
      • References for this PMC Article
        Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
      • Substance (MeSH Keyword)
        PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
      • Free in PMC
        Free full text articles in PMC
      • Cited in PMC
        Full-text articles in the PubMed Central Database that cite the current articles.

      Recent activity

      Clear Turn Off Turn On

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      You are here: NCBI > Literature > PubMed
      Write to the Help Desk