Study on spectroscopic characterization of Cu porphyrin/Co porphyrin and their interactions with ctDNA

Talanta. 2008 Jun 30;76(1):34-9. doi: 10.1016/j.talanta.2008.01.065. Epub 2008 Mar 10.

Abstract

The luminescence behaviors of Cu(II) meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin (Cu(II)TMPyP) and Co(II) meso-tetrakis (4-N-methyl-pyridiniumyl) porphyrin (Co(II)TMPyP) are investigated, and their interactions with calf thymus DNA (ctDNA) are studied by UV-vis, fluorescence and resonance light scattering (RLS) and based on the changes of UV-vis spectra, fluorescence and RLS spectra, the intrinsic binding constants of Cu(II)TMPyP/Co(II)TMPyP with ctDNA are obtained in the case of phosphate buffer solution (pH 7.2), respectively. According to the experimental results, it can be inferred that the interaction model of Cu(II)TMPyP with ctDNA is intercalative binding, while Co(II)TMPyP is the long-range assembly on the molecular surface of ctDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • DNA / chemistry*
  • Metalloporphyrins / chemical synthesis
  • Metalloporphyrins / chemistry*
  • Models, Molecular
  • Nucleic Acid Conformation
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Spectrum Analysis / methods*
  • Surface Properties

Substances

  • Metalloporphyrins
  • cobalt(III)meso-tetrakis(N-methylpyridinium-4-yl)porphyrin
  • meso-tetra(4-methylpyridinium)porphyrinato copper(II)
  • DNA
  • calf thymus DNA