Science. 2008 Aug 1;321(5889):659-63. Epub 2008 Jun 26.

Crystal structure of the termination module of a nonribosomal peptide synthetase.

Tanovic A, Samel SA, Essen LO, Marahiel MA.

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Biochemistry, Department of Chemistry, Philipps University Marburg, Hans-Meerwein-Strasse, D35032 Marburg, Germany.

Abstract

Nonribosomal peptide synthetases (NRPSs) are modular multidomain enzymes that act as an assembly line to catalyze the biosynthesis of complex natural products. The crystal structure of the 144-kilodalton Bacillus subtilis termination module SrfA-C was solved at 2.6 angstrom resolution. The adenylation and condensation domains of SrfA-C associate closely to form a catalytic platform, with their active sites on the same side of the platform. The peptidyl carrier protein domain is flexibly tethered to this platform and thus can move with its substrate-loaded 4'-phosphopantetheine arm between the active site of the adenylation domain and the donor side of the condensation domain. The SrfA-C crystal structure has implications for the rational redesign of NRPSs as a means of producing novel bioactive peptides.

PMID:: 18583577; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Structure Of Surfactin A Synthetase C (Srfa-C), A Nonribosomal Peptide Synthetase Termination Module

PDB: 2VSQ

Source: Bacillus subtilis

Method: X-Ray Diffraction

Resolution: 2.6 Å

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Crystal structure of the termination module of a nonribosomal peptide synthetase.

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