Microsatellites (triangles, solid lines). The three rightmost filled triangles denote direct estimates of the mutation rates for classes of microsatellites containing large numbers of repeats (n = 10, 8, and 3, respectively); the bottom filled triangle is a rough estimate of the mutation rate for the smallest class, taken to be the midpoint between the upper statistical bound (base of the arrow) and zero; fitted regression to all four points: (1.75 × 10−10) × L3.60 (r2 = 0.88), where L is the number of repeats. Open triangles denote estimates from two earlier studies with reporter constructs (19, 31); fitted regression: (3.24 × 10−11) × L3.42 (r2 = 0.87). Circles, squares, and dotted lines indicate homopolymeric runs. Maximum-likelihood estimates (circles) of the mutation rate of HPR loci as a function of the run length are fitted to the regression line, with each data point representing the average result over all four MA lines, with separate points for A/T and G/C runs. The regression equation is (3.73 × 10−9) × L3.78 (r2 = 0.94). The filled squares denote direct estimates derived from sequence analysis of PCR products in this study, whereas the open squares denote results from other studies (28, 29, 32); the joint regression for these latter two sets of points is (1.50 × 10−12) × L5.47 (r2 = 0.88).