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J Chromatogr A. 2008 Aug 1;1201(1):43-7. doi: 10.1016/j.chroma.2008.06.002. Epub 2008 Jun 6.

Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin.

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  • 1Viticulture, Future Farming Systems Research, Department of Primary Industries Victoria, P.O. Box 905, Mildura, Vic. 3502, Australia.


A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyanins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis.

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