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Dev Comp Immunol. 2008;32(11):1385-97. doi: 10.1016/j.dci.2008.05.008. Epub 2008 Jun 10.

B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus.

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  • 1Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA.

Abstract

B cell receptor (BCR) accessory molecules CD79a and CD79b homologs were identified in the channel catfish, Ictalurus punctatus. Both are found as single copy genes that encode proteins containing a signal peptide, an extracellular immunoglobulin domain, a transmembrane region and a cytoplasmic tail containing an immune-receptor tyrosine-dased activation motif (ITAM). IpCD79a and IpCD79b transcripts correlate well with IgM message expression. They are highly expressed in peripheral blood leukocytes (PBL) enriched in membrane (m) IgM+ cells and catfish clonal B cell lines, but not in catfish clonal T cells, indicating that IpCD79a and IpCD79b expression is B cell restricted. Studies using catfish clonal B cells (3B11) transfected with constructs encoding epitope-tagged IpCD79a and IpCD79b revealed that IpCD79a was expressed as a 45 kDa protein and IpCD79b was expressed as a 32 kDa protein. Furthermore, co-immunoprecipitations of epitope-tagged CD79 proteins demonstrate that these molecules are non-covalently associated with mIgM. These data correlate with some of the previous immunoprecipitation data demonstrating that catfish mIgM associates with proteins of 45 and 32 kDa.

PMID:
18572245
[PubMed - indexed for MEDLINE]
PMCID:
PMC2561914
Free PMC Article

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