Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype.
Lobenhofer EK,
Auman JT,
Blackshear PE,
Boorman GA,
Bushel PR,
Cunningham ML,
Fostel JM,
Gerrish K,
Heinloth AN,
Irwin RD,
Malarkey DE,
Merrick BA,
Sieber SO,
Tucker CJ,
Ward SM,
Wilson RE,
Hurban P,
Tennant RW,
Paules RS.
Cogenics, Division of Clinical Data, Inc, Morrisville, NC 27560, USA. elobenhofer@cogenics.com
This report details the standardized experimental design and the different data streams that were collected (histopathology, clinical chemistry, hematology and gene expression from the target tissue (liver) and a bio-available tissue (blood)) after treatment with eight known hepatotoxicants (at multiple time points and doses with multiple biological replicates). The results of the study demonstrate the classification of histopathological differences, likely reflecting differences in mechanisms of cell-specific toxicity, using either liver tissue or blood transcriptomic data.
PMID: 18570634 [PubMed - indexed for MEDLINE]
PMCID: PMC2481421