Monomeric and oligomeric Aβ is detected in brain extracts of humans with clinically and neuropathologically typical late-onset AD. IP/WB analysis (see Methods) was performed on supernatants of the soluble (a, TBS), membrane-associated (b, TBS-TX100) and insoluble (c, GuHCl) sequential extracts of frontal (F) and temporal (T) cortex homogenates from various individuals diagnosed with different forms of dementia (see Supp. Table 1). Samples were IP’ed with polyclonal Aβ antibody R1282 and blotted with monoclonals 2G3 (Aβ₄₀) + 21F12 (Aβ₄₂). Subject key: DS, Down’s syndrome with AD; DLB, dementia with Lewy bodies; PCD, paraneoplastic cerebellar degeneration; AD, Alzheimer’s disease; MID, multi-infarct dementia; P-AD, “pathological AD” (i.e., scattered amyloid plaques without a history of clinical AD); FTD, frontotemporal dementia. See Supp. Table 1a for further information.

Soluble Aβ extracted from AD brain alters hippocampal synapse physiology and learned behavior. (a) IP/WB of the TBS brain extracts used to study LTP. Clinical and neuropathological diagnoses for each of these seven cases are provided in Supp. Table 1b. (b) Summary data of LTP induction following two 100 Hz stimuli (HFS, arrow) of slices treated with 500 μL each of TBS vehicle (Veh; n=8 slices), Con 1 TBS extract (Con TBS; n=6) or AD 4 TBS extract (AD TBS; n=8). Insets show average baseline (light) and post-HFS (dark) fEPSP traces; calibration bars 5 msec/0.2 mV. (c) LTP is induced normally in hippocampal slices treated with 500 μL of immunodepleted AD TBS (AD TBS-ID). The summary LTP data for Con TBS (blue, n=6 slices) and AD TBS (red, n=8 slices) from Fig. 2b are represented as horizontal bars depicting means ± SEMs of fEPSP slopes at ~50–60 min post-HFS. Inset: Two sequential IP’s (R1282) of AD TBS blotted with 2G3+21F12. (d) Summary LTP data (means ± SEMs) for 3 different control subjects (Con 1, n=6; Con 2, n=6; Con 3, n=6), 4 AD subjects (AD 4 n=8; AD 5, n=5; AD 6, n=6; AD 7, n=6), 1 FTD subject (n=5) and 1 DLB subject (n=6). For comparison, LTP data from Fig. 2b for Veh are represented by the grey horizontal bar. *P<0.05 compared with Veh. (e) Summary LTD data for slices treated with 500 μL Con TBS (blue, n=7) or AD TBS (red, n=8) for 10 min prior to a weak stimulation protocol of 300 pulses at 1 Hz, indicated by the small grey bar. Calibration bars 5 msec/0.2 mV. (f) Summary LTD data for co-administration of AD TBS with either 50 μM AP-V (black, n=8), 500 μM (R/S)-MCPG (green, n=7) or 3 μM SIB1757 (orange, n=6). For comparison, LTD data (means ± SEM) for Con TBS (blue) and AD TBS (red) are shown as horizontal bars. (g) Cumulative probability distribution representing escape latency for animals receiving AD TBS (red) or AD TBS-ID (black) at 48 hr after training. Animals receiving AD TBS had a significantly shorter mean escape latency than animals receiving AD TBS-ID (174 ± 31.7 sec and 255 ± 22.8 sec, respectively; P<0.05; n=11 and 10 rats). h. Summary spine density data for pyramidal cells exposed to SEC-enriched TBS extract from AD brain (AD TBS-SEC) or from Con brain (Con TBS-SEC) and also for slices treated with 500 μM MCPG or 20 μM CPP in the presence or absence of AD TBS-SEC. *,^#P<0.05 vs. Con TBS-SEC and vs. AD TBS-SEC, respectively.

Soluble dimers are the smallest Aβ assembly form in human brain to acutely perturb synapse physiology. (a) TBS extracts of AD (top panel) and control (bottom panel) brains were subjected to non-denaturing SEC. SEC fractions were lyophilized and WB’d with 2G3+21F12; molecular weights (in kDa) on left. Note that Aβ monomer and dimer in the AD TBS extract is recovered from material that elutes at the end of the void volume (fractions 3/4). Control (Con) brain extracts were devoid of Aβ. (b) Summary LTP data (means ± SEMs) for slices treated with SEC fractions from AD or Con TBS extracts (n=6 slices for all samples), as characterized in Fig. 3a. (c) Representative WB (2G3+21F12) of IP-SEC fractionation of AD TBS and Con TBS. TBS extracts (500 μL) were immunoprecipitated with 3D6 (3 μg/mL), eluted with sample buffer and subjected to SEC. Dimer-enriched (fractions 7–8) and monomer-enriched (fractions 10–11) IP-SEC fractions were separately pooled, as were corresponding fractions from Con TBS. (d) Summary LTP data (means ± SEMs) for slices treated with IP-SEC fractions of AD and control brain TBS extracts, as characterized in Fig. 3c (Con fractions 10–11, n=5; AD fractions 10–11, n=5; Con fractions 7–8, n=7; AD fractions 7–8, n=7). (e) Mutant Aβ₄₀-S26C forms dimers under oxidizing conditions (ox), which can be reduced to monomers by treating with β-ME (red). Silver stain was performed with 100 ng wildtype Aβ₄₀ (wt) peptide or the mutant peptide (f) Summary LTP data (means ± SEMs) for slices treated with 5, 50, or 100 nM of either wt Aβ₄₀ (black) or oxidized Aβ₄₀-S26C (red) reveals that the oxidized Aβ₄₀-S26C dimer inhibits LTP with much greater potency (100 nM Aβ₄₀-S26C, n=4; n=5 for all other treatments). The vehicle controls (plotted at 0 nM) were 50 mM ammonium acetate (n=4) for the S26C peptide and 0.1% ammonium hydroxide (n=4) for wt peptide.

Insoluble amyloid cores contain Aβ dimers with synaptotoxic potential but are not readily released. (a) IP/WB of sequential extracts of the TBS-insoluble pellet prepared from 100 mg of a plaque-rich AD brain (case AD 5 from Fig. 2a) (see Methods). The final TBS washes reveal that no additional soluble Aβ can be extracted from the pellet after 4 sequential SDS washes. The remaining core-rich pellet was then incubated in formic acid (FA core extract) and analyzed by IP/WB, revealing that the insoluble cores contain Aβ monomers and dimers (far right lane). (b) Core preps following the final TBS wash as in Fig. 4a were stained with 0.2% Congo red and visualized by brightfield (left) and polarization (right) microscopy. Isolated amyloid cores display characteristic birefringence with Congo red (red arrowheads). Material prepared similarly from Con 3 (Fig. 2a) did not contain any such structures. Scale bar = 5 μm. (c) Cores prepared as in Figs. 4a, b were extracted with 88% formic acid and neutralized with NaOH. Summary LTP data for slices treated with just FA/NaOH vehicle (FA Veh, n=5), or with FA/NaOH core extracts from AD (AD core FA extract, n=7) or control (Con core FA extract, n=5) brains. Calibration bars, 5 msec/0.2 mV. (d) Summary LTP data for slices exposed to intact core preps isolated as in Fig. 4a, b from 100 mg AD cortex (AD core prep, n=5) or Con cortex (Con core prep, n=5). Calibration bars, 5 msec/0.3 mV.