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Physiol Genomics. 2008 Aug 15;34(3):225-38. doi: 10.1152/physiolgenomics.90242.2008. Epub 2008 Jun 17.

Contemporary approaches for modifying the mouse genome.

Author information

  • 1Experimental Cancer Genetics, The Wellcome Trust Sanger Institute, Hinxton, United Kingdom.

Abstract

The mouse is a premiere experimental organism that has contributed significantly to our understanding of vertebrate biology. Manipulation of the mouse genome via embryonic stem (ES) cell technology makes it possible to engineer an almost limitless repertoire of mutations to model human disease and assess gene function. In this review we outline recent advances in mouse experimental genetics and provide a "how-to" guide for those people wishing to access this technology. We also discuss new technologies, such as transposon-mediated mutagenesis, and resources of targeting vectors and ES cells, which are likely to dramatically accelerate the pace with which we can assess gene function in vivo, and the progress of forward and reverse genetic screens in mice.

PMID:
18559964
[PubMed - indexed for MEDLINE]
PMCID:
PMC2519963
Free PMC Article

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