Abstract
Oxytocin is a mammalian hormone that is released mainly after distension of the uterine cervix. In this study, we report that oxytocin stimulates intracellular release of calcium, and also activates AMPK (AMP-activated protein kinase) in C2C12 myoblast cells in a time/dose-dependent manner. Oxytocin receptor mRNA was detected in C2C12 cells. In addition, oxytocin stimulated glucose uptake and, moreover, inhibition of either CaMKK (Ca(2+)/calmodulin-dependent protein kinase kinase) or AMPK blocked oxytocin-mediated AMPK activation and glucose uptake. Taken together, our findings suggest that oxytocin may serve a peripheral metabolic function in skeletal muscle cells through the calcium-CaMKK-AMPK pathway.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
AMP-Activated Protein Kinases / metabolism*
-
Animals
-
Base Sequence
-
Biological Transport, Active / drug effects
-
Calcium / metabolism*
-
Calcium-Calmodulin-Dependent Protein Kinase Kinase / metabolism*
-
Cell Line
-
DNA Primers / genetics
-
Enzyme Activation / drug effects
-
Female
-
Glucose / metabolism*
-
Mice
-
Muscle Fibers, Skeletal / drug effects*
-
Muscle Fibers, Skeletal / metabolism*
-
Oxytocin / pharmacology*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Receptors, Oxytocin / genetics
Substances
-
DNA Primers
-
RNA, Messenger
-
Receptors, Oxytocin
-
Oxytocin
-
Calcium-Calmodulin-Dependent Protein Kinase Kinase
-
AMP-Activated Protein Kinases
-
Glucose
-
Calcium