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    J Biol Chem. 2008 Aug 1;283(31):21310-4. Epub 2008 Jun 12.

    Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28.

    Piskounova E, Viswanathan SR, Janas M, LaPierre RJ, Daley GQ, Sliz P, Gregory RI.

    Source

    Stem Cell Program, Children's Hospital Boston, the Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

    Abstract

    The developmentally regulated RNA-binding protein Lin28 blocks processing of let-7 family microRNAs (miRNAs) in embryonic cells. The molecular basis for this selective miRNA processing block is unknown. Here we find that Lin28 selectively binds the terminal loop region of let-7 precursors in vitro and that the loop mediates miRNA processing inhibition in vivo. Additionally, we identify the domains of Lin28 required for this inhibition. These findings establish a regulatory role for the terminal loop of precursors in miRNA maturation and provide insight into the mechanism by which Lin28 negatively regulates let-7 processing.

    PMID:
    18550544
    [PubMed - indexed for MEDLINE]
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