Methods Mol Biol. 2008;426:497-513.

Structural genomics of pathogenic protozoa: an overview.

Source

Department of Biochemistry, University of Washington, Seattle, WA, USA.

Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

PMID:: 18542886; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Protozoan Proteins

Grant Support

GM 064655/GM/NIGMS NIH HHS/United States

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Springer

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