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Opt Express. 2008 Feb 18;16(4):2699-708.

Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy.

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  • 1Division for Biomedical Physics, Innsbruck Medical University, Müllerstr. 44, A-6020 Innsbruck, Austria.


Wide-field Coherent Anti-Stokes Raman Scattering (CARS) microscopy is employed to identify saturated and unsaturated fatty acids in micro-emulsions and cells, using the ratio between the strong -C-H CARS signal at 2850 cm(-1) and the weak signal of the =C-H vibration around 3015 cm(-1) for distinction. Quantitative CARS imaging at the =C-H resonance is challenging, since it yields only a low CARS signal, and small differences on the order of 5% in the concentration of polyunsaturated fatty lipids have to be detected. For this purpose we draw advantage of the high signal-to-noise ratio of wide-field CARS microscopy that is achieved by an excitation geometry involving a "sheet-of-light"-type illumination.

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