Anal Biochem. 2008 Sep 1;380(1):131-3. Epub 2008 May 17.

Quantitation of human papillomavirus type 16 E6 oncogene sequences by real-time or quantitative PCR with EvaGreen.

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División de Biologi a Molecular, Instituto Potosino de Investigación Cienti fica y Tecnológica, 78216 San Luis Potosi, Mexico.

Abstract

Quantitation of E6 oncogene sequences of the human papillomavirus type 16 by real-time or quantitative PCR (qPCR) is used to determine the viral load, which correlates with the degree of the cervical neoplastic lesions. In the presence of EvaGreen, a new DNA intercalating fluorochrome, we obtained consistent and reproducible qPCR amplification curves and thermal denaturation profiles identical to those of the authentic E6-HPV16 (human papillomavirus 16) genome from the amplification products derived from a construct carrying the E6-HPV16 oncogene. E6-HPV16 quantitation in the presence of EvaGreen, therefore, is reproducible and specific and may be used to determine HPV16 viral load.

PMID:: 18539127; [PubMed - indexed for MEDLINE]

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Cited by 1 PubMed Central article

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