Virus Res. 2008 Sep;136(1-2):75-80. Epub 2008 Jun 5.

Recombinant Newcastle disease virus expressing human interleukin-2 serves as a potential candidate for tumor therapy.

Zhao H, Janke M, Fournier P, Schirrmacher V.

Source

Department of Food Science and Nutritional Engineering, China Agricultural University, 100083 Beijing, PR China. hengzhao2000@gmail.com

Abstract

A recombinant Newcastle disease virus (NDV) containing human interleukin-2 (IL-2) gene was generated by applying reverse genetics technique and further evaluated for its suitability to express and deliver IL-2 for cancer therapy. We have further analyzed the ability of rNDV/IL2 to express IL-2 in several human tumor cell lines, including the human breast carcinoma cell line MCF-7, the human colon-adenocarcinoma cell line HT29, and human Jurkat cell line. IL-2 expressed by tumor cells infected with rNDV/IL-2 was stable up till 16 days, at body temperature, and with biological activity. Expression kinetics indicated that the expression level of IL-2 was already high at 24 h after infection and reached the highest level at 48 h after infection. As NDV was proposed as a very promising oncolytic agent in a new age of therapeutic viruses, our data strongly support the application of recombinant NDV/IL-2 virus as an anti-tumor agent.

PMID:: 18538434; [PubMed - indexed for MEDLINE]

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