J R Soc Interface. 2008 Oct 6;5 Suppl 2:S123-30.

Lab-on-a-chip technologies for proteomic analysis from isolated cells.

Sedgwick H, Caron F, Monaghan PB, Kolch W, Cooper JM.

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Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, UK. hs@elec.gla.ac.uk

Abstract

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.

PMID:: 18534931; [PubMed - indexed for MEDLINE]
PMCID:: PMC2706034

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