Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Appl Immunohistochem Mol Morphol. 2008 Jul;16(4):387-92. doi: 10.1097/PAI.0b013e3181594ec6.

Use of commercially available rabbit monoclonal antibodies for immunofluorescence double staining.

Author information

  • 1Department of Pathology, Naestved Hospital, Region Sjaelland, Ringstedgade 61, Naestved 4700, Denmark. mbz@cn.stam.dk

Abstract

Immunohistochemistry, that is, the use of polyclonal and monoclonal antibodies to detect cell and tissue antigens at a microscopical level is a powerful tool for both research and diagnostic purposes. Especially in the field of hematologic disease, there is often a need to detect several antigens synchronously, and we report here a fast and easy technique for demonstrating more than 1 antigen in 1 slide using immunofluorescence. We have used commercially available rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, etc.) paired with mouse monoclonal antibodies (CD7, CD20, CD79a, Pax-5, etc.) for double immunofluorescence labeling on paraffin-embedded tissue sections. Commercially available rabbit monoclonal antibodies in combination with mouse monoclonal antibodies proved useful in double immunofluorescence labeling on paraffin-embedded tissue, and all combinations used yielded excellent results.

PMID:
18528277
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Lippincott Williams & Wilkins
    Loading ...
    Write to the Help Desk