Appl Immunohistochem Mol Morphol. 2008 Jul;16(4):387-92.

Use of commercially available rabbit monoclonal antibodies for immunofluorescence double staining.

Bzorek M, Stamp IM, Petersen BL, Frederiksen L.

Source

Department of Pathology, Naestved Hospital, Region Sjaelland, Ringstedgade 61, Naestved 4700, Denmark. mbz@cn.stam.dk

Abstract

Immunohistochemistry, that is, the use of polyclonal and monoclonal antibodies to detect cell and tissue antigens at a microscopical level is a powerful tool for both research and diagnostic purposes. Especially in the field of hematologic disease, there is often a need to detect several antigens synchronously, and we report here a fast and easy technique for demonstrating more than 1 antigen in 1 slide using immunofluorescence. We have used commercially available rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, etc.) paired with mouse monoclonal antibodies (CD7, CD20, CD79a, Pax-5, etc.) for double immunofluorescence labeling on paraffin-embedded tissue sections. Commercially available rabbit monoclonal antibodies in combination with mouse monoclonal antibodies proved useful in double immunofluorescence labeling on paraffin-embedded tissue, and all combinations used yielded excellent results.

PMID:: 18528277; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Comparative Study

MeSH Terms

Substances

Antibodies, Monoclonal

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