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J Biol Chem. 2008 Jul 25;283(30):20628-34. doi: 10.1074/jbc.M800738200. Epub 2008 Jun 4.

Synaptic vesicle protein 2 binds adenine nucleotides.

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  • 1Department of Pharmacology, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA.

Erratum in

  • J Biol Chem. 2008 Sep 5;283(36):25103.

Abstract

Synaptic vesicle protein 2 (SV2) is required for normal calcium-regulated secretion of hormones and neurotransmitters. Neurons lacking the two most widely expressed isoforms, SV2A and SV2B, have a reduced readily releasable pool of synaptic vesicles, indicating that SV2 contributes to vesicle priming. The presence of putative ATP-binding sites in SV2 suggested that SV2 might be an ATP-binding protein. To explore this, we examined the binding of the photoaffinity reagent 8-azido-ATP[gamma] biotin to purified, recombinant SV2 in the presence and absence of other nucleotides. Our results indicate that SV2A and SV2B bind nucleotides, with the highest affinity for adenine-containing nucleotides. SV2A contains two binding sites located in the cytoplasmic domains preceding the first and seventh transmembrane domains. These results suggest that SV2-mediated vesicle priming could be regulated by adenine nucleotides, which might provide a link between cellular energy levels and regulated secretion.

PMID:
18524768
[PubMed - indexed for MEDLINE]
PMCID:
PMC2475693
Free PMC Article
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