Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Rev Genet. 2008 Jul;9(7):554-66. doi: 10.1038/nrg2364. Epub 2008 Jun 3.

The art and design of genetic screens: RNA interference.

Author information

  • 1Division of Signalling and Functional Genomics, German Cancer Research Center and CBTM, University of Heidelberg, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany. m.boutros@dkfz.de

Abstract

The remarkable gene knockdown technique of RNAi has opened exciting new avenues for genetic screens in model organisms and human cells. Here we describe the current state of the art for RNAi screening, and stress the importance of well-designed assays and of analytical approaches for large-scale screening experiments, from high-throughput screens using simplified homogenous assays to microscopy and whole-animal experiments. Like classical genetic screens in the past, the success of large-scale RNAi surveys depends on a careful development of phenotypic assays and their interpretation in a relevant biological context.

PMID:
18521077
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk