Brief Funct Genomic Proteomic. 2008 Jul;7(4):312-21. Epub 2008 May 29.

The untiring search for the most complete proteome representation: reviewing the methods.

Martins de Souza D, Oliveira BM, Castro-Dias E, Winck FV, Horiuchi RS, Baldasso PA, Caetano HT, Pires NK, Marangoni S, Novello JC.

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State University of Campinas, Brazil. java99@unicamp.br

Abstract

Proteomic research has proved valuable for understanding the molecular mechanisms of biological processes, as well as in the search for biomarkers for a variety of diseases which lack a molecular diagnostic. While several new approaches are being developed, two-dimensional (2-DE) gel electrophoresis is still one of the most commonly used techniques, despite its many limitations. However, for biomarker research, 2-DE gel electrophoresis alone does not fulfill the necessary pre-requisites. If such a technique is utilized exclusively, a great part of a given proteome remains unseen. Therefore, very precise and sensitive techniques are needed. Here, we present a brief review of known methodologies that try to overcome the limitations of conventional proteome analysis as well as their respective advantages and limitations.

PMID:: 18511486; [PubMed - indexed for MEDLINE]

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