Motif positional distributions. These figures demonstrate the positional frequency of GAGA, Inr, and PB motifs. The curve shows the fraction of promoters from a given set (stalled or active) that have an instance of the motif in discrete and nonoverlapping 10-bp windows relative to the TSS, centered on multiples of 10.

Graphical representation of promoter sequences. All promoters sorted by stalling index in Toll^10b mutants. This figure demonstrates that stalled genes have a higher concentration of GAGA (red), Inr (green), and PB (black) motifs. The main section of the figure shows a horizontal line for each promoter (one pixel thick), with a colored line representing an instance of a motif. The curve on the right shows the percentage of promoters with an occurrence of each motif in a 100-promoter window as a function of stalling index. The schematic representation of a promoter (top) shows a rectangle for each motif in its modal position.

PB vs. DPE. (A) LOGO for the “Pause Button” (PB) motif, computed from instances in stalled promoters. (B) LOGO for matches to the DPE consensus (RGWYV) in stalled promoters. (C) LOGO for matches to the DPE consensus in active promoters. Stalled promoters show a DPE base composition that more closely matches the PB LOGO. Positions on the x axis of LOGOs do not represent position relative to the TSS, but show a 20-bp window around the core of each motif, centered such that positions of similar base composition match for each motif.

Predicting DV genes that have stalled Pol II. A genome browser (http://flybuzz.berkeley.edu/cgi-bin/gbrowse/fly4_3) was constructed to integrate all of the results of RNA Pol II ChIP-chip analyses performed with three 2–4 h AED mutant embryos; Toll^10b (red), Toll^rm9/rm10 (yellow), and Pipe (blue). (A, B, and D–I) Results of RNA Pol II ChIP-chip analyses on the loci of usnp (A and B), Mes2 (D and E), Wnt2 (F and G), and en (H and I). Gene prediction models are displayed above each graphical presentation. Each 3′ end is labeled with open triangle. (B, E, G, and I) Promoter sequences around transcription start site (TSS). Each TSS (+1) is underlined. GAGA element, Initiator (Inr) and Pause Button (PB)/Downstream Promoter Element (DPE) are shown in red, green and purple, respectively. (C and J) Permanganate protection assays were done for usnp (C) and engrailed (en) (J) in 2–4 h Toll^10b, Toll^rm9/rm10, and gd⁷ mutants. Transcription start sites (TSSs) are marked on the left and the location of prominent bands relative to TSS (+1) are shown on the right of the autoradiograms. Genomic sequences of G+A and C+T are shown as size markers. As a control, purified genomic DNA from yw embryos was either not treated (Naked 0″) or treated (Naked 30″ and 60″) with 20 mM KMnO₄ for 30 and 60 sec. Arrowheads indicate prominent pyrimidine residues (T or C) modified only in chromatin isolated from three mutant embryos by KMnO₄ treatment, implying the existence of transcription bubbles in vivo. (B and I) RNA Pol II ChIP-chip results and promoter sequences around TSS of usnp (B) and en (I) are shown.