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J Agric Food Chem. 2008 Jun 25;56(12):4372-6. doi: 10.1021/jf800676a. Epub 2008 May 24.

Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes.

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  • 1Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.


Soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. In previous study, we found that soybean protein hydrolysate (SPH) prepared with the protease from Bacillus amyloliquefaciens FSE-68, strongly stimulates transcription of low density lipoprotein receptor (LDL-R). To identify LDL-R transcription stimulating peptides in human hepatocytes, the SPH was fractionated with gel permeation chromatograpy and the active fraction was further separated by using reverse-phase chromatography. Several peptides in the most active fraction were identified by LC/MS and MS/MS analysis. LDL-R transcription stimulating peptides were synthesized on the basis of identified sequences, and their effect on LDL-R transcription was tested in vitro. Among the synthesized peptides, Phe-Val-Val-Asn-Ala-Thr-Ser-Asn (FVVNATSN) showed the strongest activity, and LDL-R transcription of hepatic cells was increased to 248.8% (compared to 100% of untreated control) by FVVNATSN at a concentration of 100 microM. This study provides direct evidence that peptides derived from soybean protein can influence LDL-R transcription in hepatocytes.

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