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Int J Tuberc Lung Dis. 2008 Jun;12(6):651-7.

Rapid diagnosis of Mycobacterium tuberculosis meningitis by enumeration of cerebrospinal fluid antigen-specific T-cells.

Author information

  • 1Department of Neurology, Christian Medical College, Vellore, India.

Abstract

SETTING:

Hospital in-patients with suspected tuberculous meningitis (TBM), predominantly in India.

OBJECTIVE:

To determine whether interferon-gamma (IFN-gamma) secreting Mycobacterium tuberculosis antigen-specific T-cells are present in the cerebrospinal fluid (CSF) of patients with TBM and to evaluate the feasibility of CSF enzyme-linked immunospot (ELISpot) for the diagnosis of active TBM.

DESIGN:

Prospective blinded hospital-based study.

RESULTS:

The overnight ELISpot assay detected M. tuberculosis antigen-specific IFN-gamma secreting T-cells in CSF from nine of 10 prospectively recruited patients with TBM, and zero of seven control patients with meningitis of other aetiology. This corresponds to a diagnostic sensitivity of 90% (95%CI 56-100) and specificity of 100% (95%CI 59-100).

CONCLUSION:

This pilot study demonstrates proof-of-principle for a new T-cell-based diagnostic test for TBM which is rapid, sensitive and specific.

PMID:
18492332
[PubMed - indexed for MEDLINE]
PMCID:
PMC2871295
Free PMC Article

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