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Biotechniques. 2008 May;44(6):807-9. doi: 10.2144/000112761.

A quantitative real-time PCR method for absolute telomere length.

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  • 1Commonwealth Scientific and Industrial Research Organization (CSIRO)-Human Nutrition, Adelaide, Australia. nathan.o'callaghan@csiro.au

Abstract

Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that can be used to generate absolute telomere length values rather than relative quantification. We demonstrate a strong correlation between this improved method and the "gold standard" of telomere length measurement-terminal restriction fragment analysis (TRF) by Southern hybridization. The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories.

PMID:
18476834
[PubMed - indexed for MEDLINE]
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