Nat Methods. 2008 Jun;5(6):527-9. Epub 2008 May 11.

Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.

Juette MF, Gould TJ, Lessard MD, Mlodzianoski MJ, Nagpure BS, Bennett BT, Hess ST, Bewersdorf J.

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Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.

Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

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Nat Methods. 2008 Jun;5(6):471-2.

PMID:: 18469823; [PubMed - indexed for MEDLINE]

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