J Virol Methods. 2008 Jul;151(1):158-60. Epub 2008 May 12.

Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.

Leblanc JJ, Pettipas J, Campbell SJ, Davidson RJ, Hatchette TF.

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Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada.

Abstract

Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.

PMID:: 18468698; [PubMed - indexed for MEDLINE]

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Cited by 1 PubMed Central article

Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers. [J Clin Microbiol. 2008]
Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.
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Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.

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