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J Clin Virol. 2008 Sep;43(1):102-6. doi: 10.1016/j.jcv.2008.03.025. Epub 2008 May 8.

Persistence of antibodies to West Nile virus nonstructural protein 5.

Author information

  • 1Focus Diagnostics, 5785 Corporate Avenue, Cypress, CA 90630, USA. hprince@focusdx.com

Abstract

BACKGROUND:

Because IgM antibody against West Nile virus (WNV) pre-membrane/envelope (preM/E) recombinant protein may persist for more than 1 year, an assay distinguishing recent from past WNV infection would be useful. Published findings for a single patient suggest that the presence of antibody against WNV nonstructural protein 5 (NS5) indicates recent infection.

OBJECTIVES:

To compare the persistence of WNV NS5 antibodies and preM/E IgM using plasma samples from blood donors who were viremic at the time of donation.

STUDY DESIGN:

Follow-up plasma samples from 35 viremic donors were tested for WNV NS5 antibodies using a microsphere immunoassay, and compared to WNV preM/E IgM antibodies determined on the same samples using an enzyme-linked immunosorbent assay (ELISA).

RESULTS:

At 90+/-14 days of follow-up, 20/26 donors (77%) were positive for NS5 antibodies; 6/25 (24%) were positive at 180+/-27 days, and 3/23 (13%) were positive at 365+/-55 days. The comparable values for preM/E IgM antibodies were 77%, 32% and 17%, respectively.

CONCLUSION:

Persistence of WNV NS5 antibody in plasma is similar to that of preM/E IgM antibody. WNV NS5 antibody cannot be used to distinguish recent from past WNV infection.

PMID:
18467165
[PubMed - indexed for MEDLINE]
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