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J Biomed Opt. 2008 Mar-Apr;13(2):024020. doi: 10.1117/1.2907328.

Quantitative phase microscopy through differential interference imaging.

Author information

  • 1University of Colorado, Department of Electrical and Computer Engineering, Campus Box 425 UCB Boulder, Colorado 80309-0425, USA. Sharon.king@colorado.edu

Abstract

An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.

PMID:
18465983
[PubMed - indexed for MEDLINE]
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