Development of mesh for tomographic reconstruction. a) Micro-CT image of canine hind limb section. b) Raman spectra of limb section. c) Geometric image of tissues from micro-CT data. d) Volumetric mesh created from (c). Input coordinates for reconstruction: tomographic projections and their associated scores. Illumination line (red) and collection fibers (green).

Raman tomographic images of canine bone tissue. a) Medial and anterior views of soft tissue mesh (white) and bone surface mesh of tibia and calcaneus (turquoise) overlaid with 50% contrast isosurface of the reconstructed 3-D Raman image of bone (green). b) Same view as (a) overlaid with 10% contrast isosurface of the reconstructed Raman image of bone (blue). c) 3-D mesh of limb section (white), including bone (turquoise), illustrating location of the cross-section (blue) containing the highest Raman scatter intensity. d) Raman intensity at cross-section in (c) in pseudo-color overlaid on the micro-CT image of the bone, showing range of illumination (red arrows) and collection (green dots) positions.