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J Cancer Res Clin Oncol. 2008 Nov;134(11):1219-27. doi: 10.1007/s00432-008-0398-y. Epub 2008 Apr 30.

Identification of transgelin as a potential novel biomarker for gastric adenocarcinoma based on proteomics technology.

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  • 1Key Laboratory of Infection and Oncology, Research Center of Molecular Medicine, Fujian Medical University, 88 Jiaotong Road, Fuzhou, 350004, People's Republic of China.

Abstract

PURPOSE:

To find a biomarker for gastric adenocarcinomas (GA).

METHODS:

Ten protein expression profiles of GA and paired non-neoplastic mucosa tissues were analyzed by two-dimensional gel electrophoresis. Forty-two protein spots that were differentially expressed by twofold or greater between cancer and normal mucosa tissue were excised and identified by MALDI-TOF/TOF MS. One of the over-expressed proteins identified in GA was transgelin, which was chosen for further verification by immunohistochemistry and western blotting.

RESULTS:

Forty-two distinct proteins that were differentially expressed at least twofold between the tissues were identified. Expression of 29 of these proteins was decreased (ratio >or= 2, P < 0.01), including adenosine deaminase; and 13 proteins displayed over-expression in cancer tissue (ratio >or= 2, P < 0.01), including transgelin. The results of immunohistochemistry confirmed that transgelin was indeed over-expressed in 22 cases of GA (22/41, 53.66%), with strong cytoplasmic staining in cancer cells of positive samples, this was absent in most paired non-neoplastic mucosa cells or gastric ulcer tissues (n = 20). Transgelin was found over-expressed in 21 samples of cancer tissue (21/41, 51.22%) when detected by western blot.

CONCLUSION:

This work demonstrates that differentially expressed proteins can be identified by proteomics technology combined with immunohistochemistry and western blot analyses. We have identified one such protein, transgelin, as a novel biomarker for GA.

PMID:
18446369
[PubMed - indexed for MEDLINE]
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